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sporulate vi.形成孢子。

sporulation

Israelensis recombinants , which contained recombinants plasmid pmt4 and pmt9 respectively , were obtained by electroporation . the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c . quinqnefasciatus larvae during vegetative growth stage , having the lc ? o values similar to that of . fi . sssii - 1 . however , the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed , with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9 , m txl gene from b . sphaericus was ligated with p20 and cytjaa gene , giving recombinant plasmid pmpx2 含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素,發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用;含有pmt9和pmt4的蘇云金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性,毒力與野生型b . sss - 1相當,而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異,其中b - pmt4對目標蚊幼毒力極低( lc _ ( 50 ) 10mg ml ) ,而b - pmt9對蚊幼蟲具有毒性( lc _ ( 50 ) = 2 . 49mg ml ) 。