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spermatid n.【動物;動物學】精子細胞。

spermatium

Mitochondria and ribosomes are hard to see , and the ribosome are found to adhere on the surface of endoplasmic reticulum ; golgi body and lysosome do not exist , membranous ring emerges at the secondary spermatocyte stage . the organelles change into pre - acrosome vesicles at middle spermatid stage , and finally pre - acrosome forms acrosome . 2 浙江華溪蟹在生精細胞階段,染色質形態相似,呈不同程度凝集狀,核仁在精原細胞期出現;精細胞階段,染色質分化成纖絲狀和致密塊狀,部分核質延伸入輻射臂內,整個核呈淺杯狀。

4 . influence of no on microvascular permeability : specimens from postnatal 1 day showed little immunoreactivity for enos . in contrast , blood vessel endothelium and leydig cells and spermatids in postnatal 3 weeks testes were strongly expressed enos . the number of positive blood vessel endothelium , leydig cells and spermatids in postnatal 3 months was conspicuously increased 飽及枯j代細胞均出現了陽性表達: 3月齡組生精小管靠近若腔的精r細胞出現enos陽性,陽性血管內皮細胞數目增多顯著: 12月到24月齡陽性l陽乍今內皮細胞數i ] ‘ . ‘增多的趨勢。

The number of mitochondrion is more less than the endoplasmic reticulum , and the smooth endoplasmic reticulum is the main kind of the endoplasmic reticulum ; golgi bodies and lysosomes emerge in the secondary spermatocyte stage . finally , these organelles change into pre - acrosome vesicles which become acrosome at last . sinopotamon chekiangense during the spermatogenensis , chronmatins condense at different level until middle spermatid stage 在整個發生過程中細胞器數量較少,內質網數目在各細胞器中所占比例最大,以滑面內質網為主,線粒體在初級精母細胞中最多,自次級精母細胞開始逐漸減少,高爾基體和溶酶體自次級精母細胞始出現,在發育過程中上述細胞器不斷分化,在精細胞階段形成前頂體腔,最后形成圓球形頂體。

Nucleoli exist from spermatocyte stage to early spermatid stage . in this process , the numbers of ribosomes , mitochondrions and endoplasmic reticulums increase until secondary spermatocyte stage , golgi apparatus emerges at the secondary spermatocyte stage , and then , these organelles change into lamellar complex which finally forms the acrosome 核糖體,線粒體和內質網等細胞器數量逐漸增多,到次級精母細胞階段達到最大,在形成精子的過程中,上述細胞器與高爾基體及膜性泡共同分化或參與形成片層小體,并參與頂體的形成。

In the second trial , this modified discontinuous percoll gradient centrifugation method was introduced to isolate spermatids from the semen of fifteen male infertile patients . then the effect was identified by wright - giemsa stain , flow cytometry analysis , immunocytochemistry and fluorescence in situ hybridization ( fish ) . similary , the 22 % percoll fraction contained mostly haploid cells [ ( 91 . 85 ? 5 . 18 ) % ] ( p < 0 . 005 ) and the mean density in this fraction was ( 1 . 010 ? 0 . 786 ) x 105 / ml C法,對15例各種類型不育患者的精液細胞進行分離,并利用瑞姬染色法、流式細胞術、免疫細胞化學和熒光原位雜交oisffi等方法,從細胞形態特征、 dna倍體、細胞表面標i己與分化抗原,以及原位雜交信號的數目和位置結合細胞核特有的形態等方面加以鑒定。

Nucleoli do not emerge in this process . the number of the organelles increase until secondary spermatocyte stage . mitochondria accumulate together , merging together with lysosomes and golgi bodies at the early spermatid stage , and finally the lamellar structure is formed , which forms the acrosome at last 在精子發生過程中,線粒體、內質網和核糖體逐漸增多,其中線粒體數目在次級精母細胞階段達到頂峰,并形成線粒體區,精細胞早期核內出現膜性泡結構,同時次級溶酶體與高爾基體大量存在,這些細胞器共同形成片層復合體,并參與頂體的形成。

The first reports on successful clinical use of round and elongated spermatids for assisted reproduction in 1995 suggest the benefits that the technique of spermatid conception can bring to some patients suffering from otherwise untreatable types of male factor infertility . men with non - obstructive azoospermia ( noa ) can now be treated by using intra - oocyte round spermatid injection ( rosi ) or elongated spermatid injection ( elsi ) . spermatids can be retrieved from semen or from testis biopsy specimens 目前,完全不能產生精子的非梗阻性無精子癥( non - obstmctiveazoospermia , noa )患者可采用卵胞質內圓形精子細胞注射( roundspermatidinjection , rosi )或長形精子細胞注射( elongatedspermatidinection , elsi )治療,精子細胞可從精液或從睪丸活檢組織中獲取。

In the first trial , combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis , and then a modified discontinuous percoll gradient centrifugation method ( 15 % , 22 % , 30 % , 40 % , 50 % , 60 % ) was introduced to isolate spermatids from the cellular suspensions . the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis , and the viability of spermatogenic cells was assessed by using eosin y exclusion test 在第一部分試驗中,首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液,然后經6層非連續percoll梯度離心法( 15 、 22 、 30 、 40 、 50和60 )分離,通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量,并以伊紅y排斥試驗測定細胞的存活率。

Except the incomplete maturation of spermatid nuclear and oocyte activation , idendification of a live spermatid is a pivotal procedure . it is difficult to distinguish round spermatids from other round cells such as spermatocytes , monocytes , polymorphonuclear leukocytes and so on 除精子細胞的核蛋白不完全成熟及卵子激活不足等因素外,如何正確選擇存活精子細胞是個難題,如圓形精子細胞與精母細胞、單核細胞和多形核白細胞等其他圓形細胞的區分就比較困難。

There are five developmental stages in the spermatogenesis , namely are primary spermatogonium , secondary spermatogonium , primary spermatocytes , secondaty spermatocytes and spermatids . the spermatids develop into sperm via the process of spermiogenesis . there exist cell junctions between two spermatids 電子顯微鏡觀察表明:大眼鱖的精子發生和形成過程,經歷了初級精原細胞,次級精原細胞,初級精母細胞,次級精母細胞和精子細胞階段。

Most commonly used is the mechanical method in which a small piece of testicular tissue is torn apart using needles and the spermatids are released . while enzymatic digestion of human testicular tissue can result in a mixture of cell types including a whole range of spermatogenic cell types 最常用的是機械方法,即利用細針將活檢獲得的小塊睪丸組織撕開,釋放出精子細胞;也可利用酶將睪丸組織消化成細胞懸液,其中包含所有類型的生精細胞。

Portunus , trituberculatus the spermatogonium exist in two different forms , the second form comes from the first one . during spermatogenesis , chromatins condense at different level . the nucleus changes into homogeneous since middle spermatid stage , and the nucleus extends into the radial arm 三疣梭子蟹在生精細胞階段,染色質不同程度的濃縮凝集;在精細胞階段,核質呈均質狀,形成薄壁的圓球形核杯,部分核質延伸入輻射臂內。

According to the changes of the structure of chromatin and the morphology of cell , the process of the typical spermatogenesis of cipangopaludina chinensis can be divided into five stages : spermatogonium , primary spermatocyte , secondary spermatocyte , spermatid and mature sperm 中國圓田螺典型精子發生時,根據染色質和細胞形態的變化,可將其發生分為以下五個時期:精原細胞、初級精母細胞、次級精母細胞、精細胞和成熟精子。

Therefore there is a need to develop a method by which large and purified populations of spermatids can be isolated , not only for the immediate requirement but also for cryoproservation for subsequent treatment cycles 因此,有必要建立一種比較簡單的、可獲取大量純化精子細胞的方法,不但可滿足立即使用所需,也可進行冷凍保存用于以后的治療周期。

The nucleus has two forms at the middle spermatid stage : fibrous one and condensed one . the nucleus extends into the radial arm . the number of organelles keeps increasing until secondary spermatocyte stage 與已有的報導不同的是,我們發現精原細胞分為兩類,其中i類精原細胞經過分化形成n類精原細胞。

However , the rates of fertilization and pregnancy with spermatids have been disappointing . many problems limiting success rates and binding a wide application of this technique still remain unresolved 但精子細胞的受精率和妊娠率令人失望,限制這項技術成功和阻礙其臨床應用的許多問題仍然沒有解決。

So a lagre populations of relatively purified spermatids can be isolated from mouse testis by enzymatic digestion combined with discontinuous percoll gradient centrifugation method 因此采用組合酶消化結合非連續percoll梯度離。 g法,可有效地從小鼠睪丸中分離到較多較純的存活精子細胞。

Spermatogenesis macrobrachium rosenbergii during spermatogenesis , chromatins keep on condensing until mid - spermatid stage . the nucleus changes into two forms : fibrous and homogeneous forms 精子發生羅氏沼蝦精子發生過程中染色質凝集程度逐漸增大,至精細胞中期凝集程度達到最大。

A variety of biology events occur during spermatogenesis , such as self - renewal of spermatogonia , meiotic division of spermatocyte and the spermiogenesis of spermatid 生精過程中發生了許多生物學的變化,如精原細胞的自我更新,精母細胞的減數分裂和精子細胞的變形。