C - clon 中文 - EN166

clon n.1.【生物學】純種細胞，無性系。2.復制品；(幾乎)...

Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l . cv hetao flesh , which was cloned into plasmid vector pmd - 18 - t . the clon of antisense orientation were selected , and it was inserted downstream of camv35s promoter and enhancer “ “ of tmv into the plant expression vector pbinyxw , antisence expression vector pbinya was constructed . at the base that pollination and fertilization of cucumis melo l . cv hetao was studied , using pollen tube pathway transformate cucumis melo l . cv hetao , 76 fruit had been obtained , moreover , hardness and content of sugar were analysed 本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然后將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子“ ”的下游，構建成反義表達載體pbinya 。并在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，并進行了硬度和含糖量的分析。
clonal
The data above showed that algae phycobiliprotein fluorescent probes not only facilitate the study to pea actins but also provide a doable method to label other plant proteins . it has a good foreground for algae fluorescence probe to be used in the immunity fluorescent detection of plant cells . the gene clon 對豌豆肌動蛋白藻熒光探針的初步研究，不僅為豌豆肌動蛋白的免疫熒光檢測提供了便利條件，而且通過改變抗體，則可以將藻熒光探針用于其他植物蛋白的標記，藻熒光探針優越的熒光特性使其在植物材料的免疫熒光檢測中具有廣闊的應用前景。
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l . cv hetao flesh , which was cloned into plasmid vector pmd - 18 - t . the clon of antisense orientation were selected , and it was inserted downstream of camv35s promoter and enhancer “ “ of tmv into the plant expression vector pbinyxw , antisence expression vector pbinya was constructed . at the base that pollination and fertilization of cucumis melo l . cv hetao was studied , using pollen tube pathway transformate cucumis melo l . cv hetao , 76 fruit had been obtained , moreover , hardness and content of sugar were analysed 本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然后將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子“ ”的下游，構建成反義表達載體pbinya 。并在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，并進行了硬度和含糖量的分析。